Effect of therapeutic hypercapnia on the early inflammatory factors after ischemia-reperfusion injury of lung transplantation
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摘要:
目的 研究治疗性高碳酸血症对肺移植缺血-再灌注早期炎症因子的影响。 方 法 72只Wistar雄性大鼠,随机分为模型组(36只)和治疗组(36只)。两组基础状态给予50% N2+50% O2通气,模型组移植成功后给予50% N2+50% O2通气;治疗组移植成功后给予50% O2、8%CO2和42% N2通气,维持动脉血二氧化碳分压(PaCO2)在80~100 mmHg(10 mmHg=1.33 kPa)之间。记录受体大鼠机械通气1 min时MAP、PaCO2和动脉血氧分压(PaO2)作为基础值,再灌注期间每30 min记录1次,直至实验结束。分别于再灌注1、2、4 h取左肺下叶组织,采用酶联免疫吸附法测定肺组织中肿瘤坏死因子(TNF)-α和白细胞介素(IL)-1β的水平。 结果 与模型组比较,再灌注后各时间点治疗组受体鼠的MAP、PaO2均明显升高(均为P<0.05)。与模型组比较,治疗组各时间点移植肺组织中TNF-α和IL-1β水平均有显著下降(均为P<0.05)。 结论 治疗性高碳酸血症对肺移植缺血-再灌注损伤后早期炎症因子的释放有抑制作用。 Abstract:Objective To investigate the effect of therapeutic hypercapnia on the early inflammatory factors after ischemia-reperfusion of lung transplantation. Method Seventy-two male Wistar rats were randomly divided into model group (n=36) and therapy group (n=36). Rats on the baseline in model group and therapy group were ventilated with 50% N2 and 50% O2. Rats in model group were continuously ventilated with 50% N2 and 50% O2 after successful transplantation. Rats in therapy group were ventilated with mixed gases after successful transplantation, which included 50% O2, 8% CO2, and 42% N2 to keep arterial partial pressure of carbon dioxide(PaCO2) in the range of 80~100 mmHg(10 mmHg=1.33 kPa). The mean arterial pressure (MAP)、PaCO2 and arterial partial pressure of oxygen (PaO2) of recipient rats were recorded as baseline value after mechanical ventilation for 1 minute. Then the data were recorded once every 30 minutes during reperfusion period until the end. The inferior lobes of left lung sample were taken at 1, 2,4 h after reperfusion respectively. The levels of tumor necrosis factor(TNF)-α and interleukin(IL)-1β in lung tissues were measured by enzyme-linked immunosorbent assay. Result Compared with model group, MAP and PaO2 were significantly higher in therapy group at different time points after reperfusion (all in P<0.05). Compared with model group, the levels of TNF-α and IL-1β of transplant lung tissues were significantly lower in therapy group at different time points after reperfusion(all in P<0.05). Conclusion The therapeutic hypercapnia plays an inhibitive role on the release of early inflammatory factors after the ischemia-reperfusion injury of lung transplantation. -
表 1 两组受体鼠再灌注后不同时间点平均动脉压与血气分析结果的比较
Table 1. Comparison of mean arterial pressure and arterial blood gas analysis of recipient rats between two groups at different time points after reperfusion (mmHg,x±s)
项目 n 基础值 再灌注 30 min 60 min 90 min 120 min 150 min 180 min 210 min 240 min MAP 治疗组模型组 1212 110±10115±8 112±8b95±10a 113±12b97±8a 118±10b100±7a 117±12b102±11a 114±12b106±12a 112±10b103±13a 114±12b100±11a 112±14b99±11a PaO2 治疗组模型组 1212 234±19231±15 218±36b185±30a 225±30b186±26a 232±32b183±30a 240±38b178±38a 242±33b176±40a 245±32b174±42a 241±35b170±36a 247±31b168±42a PaCO2 治疗组模型组 1212 31±434±3 82±5a,b37±4 84±7a,b39±5 90±8a,b42±6 87±5a,b40±5 89±8a,b44±3 88±6a,b46±4 85±5a,b43±4 82±7a,b42±5 注:与同组基础值比较,aP<0.05;与模型组比较,bP<0.05 表 2 两组受体鼠再灌注后移植肺组织炎症因子的比较
Table 2. Comparison of inflammatory factors of transplant lung tissues of recipient rats between two groups after reperfusion (μg/L,x±s)
组别 n TNF-α IL-1β 再灌注1 h 再灌注2 h 再灌注4 h 再灌注1 h 再灌注2 h 再灌注4 h 治疗组 12 0.50±0.11a 0.62±0.06a 0.77±0.14a 0.28±0.03a 0.33±0.06a 0.31±0.05a 模型组 12 0.76±0.05 0.82±0.05 0.89±0.16 0.32±0.05 0.37±0.05 0.33±0.07 注:与模型组比较,aP<0.05 -
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