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过继回输耐受性树突状细胞促进大鼠肝移植免疫耐受的应用研究

贾亚男, 周林, 赵阳, 等. 过继回输耐受性树突状细胞促进大鼠肝移植免疫耐受的应用研究[J]. 器官移植, 2022, 13(3): 371-377. doi: 10.3969/j.issn.1674-7445.2022.03.014
引用本文: 贾亚男, 周林, 赵阳, 等. 过继回输耐受性树突状细胞促进大鼠肝移植免疫耐受的应用研究[J]. 器官移植, 2022, 13(3): 371-377. doi: 10.3969/j.issn.1674-7445.2022.03.014
Jia Ya' nan, Zhou Lin, Zhao Yang, et al. Application study on adoptive transfusion of tolerogenic dendritic cells in promoting immune tolerance of liver transplantation in rat models[J]. ORGAN TRANSPLANTATION, 2022, 13(3): 371-377. doi: 10.3969/j.issn.1674-7445.2022.03.014
Citation: Jia Ya' nan, Zhou Lin, Zhao Yang, et al. Application study on adoptive transfusion of tolerogenic dendritic cells in promoting immune tolerance of liver transplantation in rat models[J]. ORGAN TRANSPLANTATION, 2022, 13(3): 371-377. doi: 10.3969/j.issn.1674-7445.2022.03.014

过继回输耐受性树突状细胞促进大鼠肝移植免疫耐受的应用研究

doi: 10.3969/j.issn.1674-7445.2022.03.014
基金项目: 

北京市自然科学基金 7212042

首都医科大学附属北京朝阳医院科技创新基金 21kcjj-4

详细信息
    作者简介:
    通讯作者:

    贺强,男,1964年生,博士,主任医师,研究方向为肝移植与肝胆胰恶性肿瘤,Email:heqiang349@sina.com

    李先亮,男,1971年生,博士,副主任医师,研究方向为肝移植的基础与临床研究,Email:lixianliangbjcy@126.com

  • 中图分类号: R617, R392.4

Application study on adoptive transfusion of tolerogenic dendritic cells in promoting immune tolerance of liver transplantation in rat models

More Information
  • 摘要:   目的  探讨耐受性树突状细胞(tolDC)在肝移植免疫耐受诱导中的作用。  方法  建立自发耐受[Brown Norway(BN)→Lewis,耐受组,n=6]和急性排斥反应(AR)(Lewis→BN)大鼠肝移植模型,AR模型大鼠中实验组进行tolDC回输(tolDC组,n=6),对照组不进行干预(AR组,n=6)。观察各组大鼠术后生存时间,对各组大鼠移植肝组织进行病理学检查;采用流式细胞术检测各组大鼠外周血、移植肝、脾脏、淋巴结髓样树突状细胞(mDC)和浆细胞样树突状细胞(pDC)的表达情况;采用酶联免疫吸附试验检测各组大鼠血清白细胞介素(IL)-10和干扰素(IFN)-γ的表达情况。  结果  AR组大鼠病理学表现主要为移植肝炎症细胞浸润和组织结构紊乱,生存时间7~14 d;tolDC组与耐受组大鼠移植肝组织基本正常,最长生存时间超过100 d。与AR组比较,耐受组和tolDC组大鼠外周血、移植肝、脾脏、淋巴结CD11+mDC水平均下降(均为P < 0.05),且CD11+mDC表面CD86、主要组织相容性复合体(MHC)Ⅱ表达水平均降低(均为P < 0.05)。与AR组比较,耐受组和tolDC组大鼠外周血、移植肝、脾脏、淋巴结中pDC水平均升高(均为P < 0.05),同时pDC表面的MHCⅡ表达水平均降低(均为P < 0.05)。与AR组比较,耐受组和tolDC组大鼠血清IL-10的表达水平均升高、IFN-γ的表达水平均降低(均为P < 0.05)。  结论  作为tolDC亚群,mDC和pDC在大鼠肝移植术后移植物免疫耐受的发生过程中均具有正向调节作用。

     

  • 图  1  各组大鼠肝组织病理学染色结果

    注:A图为AR组;B组为耐受组;C图为tolDC组(HE,×100)。

    Figure  1.  Pathological staining results of liver tissue of rats in each group

    图  2  各组大鼠CD11+mDC的分析结果

    注:A图为mDC的流式检测示意图;B图为各组大鼠不同组织中CD11+mDC表达水平;与AR组比较,aP < 0.05。

    Figure  2.  Analysis results of CD11+mDC of rats in each group

    图  3  各组大鼠CD11+mDC表面CD86和MHC Ⅱ的表达水平

    注:A图为各组大鼠不同组织中CD11+mDC表面CD86的表达水平;B图为各组大鼠不同组织中CD11+mDC表面MHCⅡ的表达水平;与AR组比较,aP < 0.05。

    Figure  3.  Expression levels of CD86 and MHC Ⅱ on the surface of CD11+mDC of rats in each group

    图  4  各组大鼠不同组织中pDC及其表面MHC Ⅱ的表达水平

    注:A图为各组大鼠不同组织中pDC表达水平;B图为各组大鼠不同组织中pDC表面MHCⅡ的表达水平;与AR组比较,aP < 0.05。

    Figure  4.  Expression levels of pDC and MHC Ⅱ on its surface in different tissues of rats in each group

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出版历程
  • 收稿日期:  2022-01-14
  • 网络出版日期:  2022-04-29
  • 刊出日期:  2022-05-15

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